ABSTRACT
Age at death estimation has always been a crucial yet challenging part of identification process in forensic field. The use of human skeletons have long been explored using the principle of macro and micro-architecture change in correlation with increasing age. The clavicle is recommended as the best candidate for accurate age estimation because of its accessibility, time to maturation and minimal effect from weight. Our study applies pre-trained convolutional neural network in order to achieve the most accurate and cost effective age estimation model using clavicular bone. The total of 988 clavicles of Thai population with known age and sex were radiographed using Kodak 9000 Extra-oral Imaging System. The radiographs then went through preprocessing protocol which include region of interest selection and quality assessment. Additional samples were generated using generative adversarial network. The total clavicular images used in this study were 3,999 which were then separated into training and test set, and the test set were subsequently categorized into 7 age groups. GoogLeNet was modified at two layers and fine tuned the parameters. The highest validation accuracy was 89.02% but the test set achieved only 30% accuracy. Our results show that the use of medial clavicular radiographs has a potential in the field of age at death estimation, thus, further study is recommended.
ABSTRACT
Stature is an essential component of biological profile analysis since it determines an individual’s physical identity. Long bone dimensions are generally used to estimate the stature of skeletal remains; however, non-long bones such as the sternum, cranium, and sacrum may be necessary for some forensic situations. This study aimed to generate a regression equation for stature estimation of the skeletal remains in the Thai population. Ten measurements of the sacrum were measured from 200 dry sacra. The results revealed that the maximum anterior breadth (MAB) provided the most accurate stature prediction model among males (correlation coefficient [r]=0.53), standard error of estimation (SEE=5.94 cm), and females (r=0.48, SEE=6.34 cm). For the multiple regression model, the best multiple regression models were stature equals 41.2+0.374 (right auricular surface height [RASH])+1.072 (anterior-posterior outer diameter of S 1 vertebra corpus [APOD])+0.256 (dorsal height [DH])+0.417 (transverse inner diameter of S 1 vertebra corpus [TranID])+0.2 (MAB) with a SEE of 6.42 cm for combined sex. For males, stature equals 63.639+0.478 (MAB)+0.299 (DH)+0.508 (APOD) with a SEE of 5.35, and stature equals 75.181+0.362 (MAB)+0.441 (RASH)+0.132 (maximum anterior height [MAH]) with a SEE of 5.88 cm for females. This study suggests that regression equations derived from the sacrum can be used to estimate the stature of the Thai population, especially when a long bone is unavailable.
ABSTRACT
Stature estimation is an important process of biological profile analysis for the identification of skeletal remains.In forensic practice, non-long bones might be needed for estimating stature, in case long bones were not recovered or well preserved. This study developed the stature estimation model from dry sterna in a Thai population. The combined length of manubrium and mesosternum (CMM) was the best single stature estimation indicator for males and combined sex samples, whereas the sternal area (SA) was the best stature estimation indicator for females in our study. The best multiple regression analysis models of our study were stature equal 122.685+0.182 (CMM)+0.592 (intercostal length between the third and fourth ribs [ICL34 ]) with a standard error of estimation (SEE) of 6.134 cm for males, stature equal 130.676+0.005 (SA) with SEE of 5.370 cm for females, and stature equal 79.412+0.342 (CMM)+0.506 (corpus sterni width at first sternebra [CSWS1 ])+0.794 (ICL34 ) with SEE of 6.222 cm for unknown sex samples. The results indicated that a sternum can be used for estimating the stature of skeletal remains in a Thai population. However, these models might not be suitable for other populations, especially, in case the suspected stature is over the stature range in our study.
ABSTRACT
The estimation of sex is an essential component of forensic osteological analyses, and the potential of an incomplete radius for sex determination of human remains is investigated. The present study was conducted on 200 left-right pairs of radial bone from a northern Thai population (100 males and 100 females). The most dimorphic single parameter was maximum head diameter (MDH) with accuracies 92.0% for the right side and 90.5% for the left side. At the distal part of radius, the distal end width of the radius (RDEW) was the best sex indicator, in which the sex classification accuracies were 91.5% and 89.0%, for the right and left sides, respectively. Stepwise discriminant function analysis was performed for all measurements and specified separately to the proximal and distal radius. The circumference of the radial neck, headtuberosity length, MDH, and RDEW were selected for the stepwise procedure as these parameters produced the best correct classification results for both sides. The use of proximal radius for sex estimation was examined, with accuracies of 95.0% and 93.0% for the right and left sides, respectively. The sex classification functions for distal radius provided the accuracies of 92.5% and 89.5%, for the right and left sides, respectively. In summary, the fragments of radius indicated a high ability to estimate sex in the Northern Thai population.
ABSTRACT
Ischemic stroke caused by occlusion of cerebral artery is responsible for the majority of stroke that increases the morbidity and mortality worldwide. Diabetes mellitus (DM) is a crucial risk factor for ischemic stroke. Prolonged DM causes various microvascular and macrovascular changes, and blood-brain barrier (BBB) permeability that facilitates inflammatory response following stroke. In the acute phase following stroke, BBB disruption has been considered the initial step that induces neurological deficit and functional disabilities. Stroke outcomes are significantly worse among DM. In this article, we review stroke with diabetes-induce BBB damage, as well as underlying mechanism and possible therapeutic targets for stroke with diabetes.
ABSTRACT
The estimation of sex is an essential component of forensic osteological analyses, and the potential of an incomplete radius for sex determination of human remains is investigated. The present study was conducted on 200 left-right pairs of radial bone from a northern Thai population (100 males and 100 females). The most dimorphic single parameter was maximum head diameter (MDH) with accuracies 92.0% for the right side and 90.5% for the left side. At the distal part of radius, the distal end width of the radius (RDEW) was the best sex indicator, in which the sex classification accuracies were 91.5% and 89.0%, for the right and left sides, respectively. Stepwise discriminant function analysis was performed for all measurements and specified separately to the proximal and distal radius. The circumference of the radial neck, headtuberosity length, MDH, and RDEW were selected for the stepwise procedure as these parameters produced the best correct classification results for both sides. The use of proximal radius for sex estimation was examined, with accuracies of 95.0% and 93.0% for the right and left sides, respectively. The sex classification functions for distal radius provided the accuracies of 92.5% and 89.5%, for the right and left sides, respectively. In summary, the fragments of radius indicated a high ability to estimate sex in the Northern Thai population.
ABSTRACT
Ischemic stroke caused by occlusion of cerebral artery is responsible for the majority of stroke that increases the morbidity and mortality worldwide. Diabetes mellitus (DM) is a crucial risk factor for ischemic stroke. Prolonged DM causes various microvascular and macrovascular changes, and blood-brain barrier (BBB) permeability that facilitates inflammatory response following stroke. In the acute phase following stroke, BBB disruption has been considered the initial step that induces neurological deficit and functional disabilities. Stroke outcomes are significantly worse among DM. In this article, we review stroke with diabetes-induce BBB damage, as well as underlying mechanism and possible therapeutic targets for stroke with diabetes.
ABSTRACT
Mandibular foramen (MF) is a structure that inferior alveolar nerve and artery pass through itself which is found on the mandible. The objective of this research aims to locate MF among the Thai population including other MF characteristics. The sampling is conducted in the Thai population of the total number of 220 samples from the Forensic Osteology Research Center, Faculty of Medicine, Chiang Mai University. The MF anteroposterior and superoinferior localizations are similar in both sex which at Q3 of right anteroposterior, Q2 of left anteroposterior and Q2 of left and right superoinferior axis. Otherwise, the prevalence of accessory MF has in 68 samples which are 30.89%. The prevalence of unilateral single accessory MF is 20.45%, bilateral single accessory MF is 6.36%, unilateral double accessory MF is 2.27% and bilateral double accessory MF is 0.45%. This present research results that the Thai population has a difference in MF location while comparing to other populations. Moreover, Thai MF and accessory MF location and localization will be helpful to clinical implications.
ABSTRACT
The human heart valves are complex anatomical structures consisting of leaflets with many supporting structures. With advancing age, the microstructure of the components of the valves can change. Knowledge and understanding of the anatomical relationships between the different components of the heart valve structures and their relationship with age is crucial for the development and progression of treatment of valvular disease. The purpose of this study was to determine histological changes of the components of the heart valves and their relationship with age. Fifty hearts taken from cadavers were included to examine the histology of the tricuspid, mitral, pulmonary, and aortic valves. All specimens were stained with Elastic Van Gieson, and picrosirius red to enable the evaluation of elastic and collagen fibers, respectively. There was a gradual increase in elastic and collagen fibers with advancing age, particularly over 40 years, in all valve types. In the case of tricuspid and mitral valves increases in collagen and elastic fibers were observed starting in the fifth decade. Elastic fiber fragmentation was observed in specimens over 50 years. In the case of the pulmonary and the aortic valves, collagen fibers were denser and more irregular in the sixth to seventh decades when compared to younger ages while elastic fibers were significantly increased in the sixth decade. In addition, an increase in fat deposition had an association with aging. These findings provide additional basic knowledge in age-related morphological changes of the heart valves and will increase understanding concerning valvular heart diseases and treatment options.
ABSTRACT
Mandibular foramen (MF) is a structure that inferior alveolar nerve and artery pass through itself which is found on the mandible. The objective of this research aims to locate MF among the Thai population including other MF characteristics. The sampling is conducted in the Thai population of the total number of 220 samples from the Forensic Osteology Research Center, Faculty of Medicine, Chiang Mai University. The MF anteroposterior and superoinferior localizations are similar in both sex which at Q3 of right anteroposterior, Q2 of left anteroposterior and Q2 of left and right superoinferior axis. Otherwise, the prevalence of accessory MF has in 68 samples which are 30.89%. The prevalence of unilateral single accessory MF is 20.45%, bilateral single accessory MF is 6.36%, unilateral double accessory MF is 2.27% and bilateral double accessory MF is 0.45%. This present research results that the Thai population has a difference in MF location while comparing to other populations. Moreover, Thai MF and accessory MF location and localization will be helpful to clinical implications.
ABSTRACT
Image analysis has an increasing role in the identification of individuals in forensic application. Beside the bones, microstructural of arteries can be used in age estimation study. Aorta is the largest elastic artery which consists of many elastic fibers. Elastin in arterial wall highly resist to chemical and physical influence. The purposes of the study were to quantify elastic fibers in tunica media in each location of the aorta and examine the correlation between elastic fibers and age by using image analysis program. A total of 36 human aortas were dissected in 4 locations. The aortas were obtained from cadavers with an age range of 20 to 90 years. Specimens were stained with Elastic Van Gieson staining. Histological images were investigated about elastic fibers using light microscope with cellSens program and aorta image analysis was used for the evaluation of data. The results showed that the mean percentage density of elastic fibers in the ascending aorta and the aortic arch increased. However, the mean percentage density of elastic fibers decreased in the 31 to 40 years age group in the thoracic aorta and the abdominal aorta and decreased in each location of aorta continuously until 81 to 90 years. The abdominal aorta showed the highest correlation with age (r=0.732) followed by the thoracic aorta, the aortic arch and the ascending aorta, respectively. Changes in the percentage density of elastic fibers in the tunica media of the aortic wall can be used to add information to age estimation for identification purposes.
ABSTRACT
The human heart valves are complex anatomical structures consisting of leaflets with many supporting structures. With advancing age, the microstructure of the components of the valves can change. Knowledge and understanding of the anatomical relationships between the different components of the heart valve structures and their relationship with age is crucial for the development and progression of treatment of valvular disease. The purpose of this study was to determine histological changes of the components of the heart valves and their relationship with age. Fifty hearts taken from cadavers were included to examine the histology of the tricuspid, mitral, pulmonary, and aortic valves. All specimens were stained with Elastic Van Gieson, and picrosirius red to enable the evaluation of elastic and collagen fibers, respectively. There was a gradual increase in elastic and collagen fibers with advancing age, particularly over 40 years, in all valve types. In the case of tricuspid and mitral valves increases in collagen and elastic fibers were observed starting in the fifth decade. Elastic fiber fragmentation was observed in specimens over 50 years. In the case of the pulmonary and the aortic valves, collagen fibers were denser and more irregular in the sixth to seventh decades when compared to younger ages while elastic fibers were significantly increased in the sixth decade. In addition, an increase in fat deposition had an association with aging. These findings provide additional basic knowledge in age-related morphological changes of the heart valves and will increase understanding concerning valvular heart diseases and treatment options.
ABSTRACT
Image analysis has an increasing role in the identification of individuals in forensic application. Beside the bones, microstructural of arteries can be used in age estimation study. Aorta is the largest elastic artery which consists of many elastic fibers. Elastin in arterial wall highly resist to chemical and physical influence. The purposes of the study were to quantify elastic fibers in tunica media in each location of the aorta and examine the correlation between elastic fibers and age by using image analysis program. A total of 36 human aortas were dissected in 4 locations. The aortas were obtained from cadavers with an age range of 20 to 90 years. Specimens were stained with Elastic Van Gieson staining. Histological images were investigated about elastic fibers using light microscope with cellSens program and aorta image analysis was used for the evaluation of data. The results showed that the mean percentage density of elastic fibers in the ascending aorta and the aortic arch increased. However, the mean percentage density of elastic fibers decreased in the 31 to 40 years age group in the thoracic aorta and the abdominal aorta and decreased in each location of aorta continuously until 81 to 90 years. The abdominal aorta showed the highest correlation with age (r=0.732) followed by the thoracic aorta, the aortic arch and the ascending aorta, respectively. Changes in the percentage density of elastic fibers in the tunica media of the aortic wall can be used to add information to age estimation for identification purposes.
ABSTRACT
The rupture of ligament in the lateral part of ankle joint is a common injury and can lead to chronic ankle instability and lead to ankle osteoarthritis. Ankle arthroscopy is considered as a standard option to treat various ankle problems due to the need for only minimal incisions and fewer complications when compared to open surgery. However, there are complications associated with arthroscopic surgery e.g. damage to the anatomical structures around the portal placement areas. The present review provides anatomical knowledge of the superficial and deep neurovascular structures in the ankle region. These structures are important when ankle surgery is performed in order to avoid any intraoperative injury and prevent any complication following surgery.
ABSTRACT
Iatrogenic injury to subcutaneous nerves on the dorsum of the foot is an established risk factor during the surgical procedures resulting in postoperative pain, sensation loss and painful neuroma. Previous studies have reported on the distribution pattern of the superficial peroneal, deep peroneal and sural nerves (SNs) and their branches with various classifications about specific populations. The purpose of the present study was to evaluate the distribution pattern and classification of the nerves on the dorsum of the foot and analyze the location of these nerves into five zones with clinical implications. Fifty-four lower limbs of fresh frozen cadavers were used in the present study. The anatomical patterns of the superficial peroneal, deep peroneal, SN and their branches were classified into eight, two and five patterns respectively. Type VI pattern, a classic distribution pattern of the superficial peroneal nerve was the most frequent type with a prevalence of 13 limbs (25.00%). In Zone 5, where the arthroscopic portal placements for the first metatarsophalangeal joint arthrodesis, 29 limbs (55.77%) showed three nerve branches located in this zone. The results of the present study provide anatomical knowledge that may help the surgeon to choose the appropriate treatment for their patients and reducing the rate of complications in surgery.
ABSTRACT
The rupture of ligament in the lateral part of ankle joint is a common injury and can lead to chronic ankle instability and lead to ankle osteoarthritis. Ankle arthroscopy is considered as a standard option to treat various ankle problems due to the need for only minimal incisions and fewer complications when compared to open surgery. However, there are complications associated with arthroscopic surgery e.g. damage to the anatomical structures around the portal placement areas. The present review provides anatomical knowledge of the superficial and deep neurovascular structures in the ankle region. These structures are important when ankle surgery is performed in order to avoid any intraoperative injury and prevent any complication following surgery.
ABSTRACT
Iatrogenic injury to subcutaneous nerves on the dorsum of the foot is an established risk factor during the surgical procedures resulting in postoperative pain, sensation loss and painful neuroma. Previous studies have reported on the distribution pattern of the superficial peroneal, deep peroneal and sural nerves (SNs) and their branches with various classifications about specific populations. The purpose of the present study was to evaluate the distribution pattern and classification of the nerves on the dorsum of the foot and analyze the location of these nerves into five zones with clinical implications. Fifty-four lower limbs of fresh frozen cadavers were used in the present study. The anatomical patterns of the superficial peroneal, deep peroneal, SN and their branches were classified into eight, two and five patterns respectively. Type VI pattern, a classic distribution pattern of the superficial peroneal nerve was the most frequent type with a prevalence of 13 limbs (25.00%). In Zone 5, where the arthroscopic portal placements for the first metatarsophalangeal joint arthrodesis, 29 limbs (55.77%) showed three nerve branches located in this zone. The results of the present study provide anatomical knowledge that may help the surgeon to choose the appropriate treatment for their patients and reducing the rate of complications in surgery.
ABSTRACT
Sex estimation from skeletal remains is an important practice for forensic human identification. The aims of this study were to assess sexual dimorphism and to develop discriminant function equations for sex estimation using 12 measurements of upper limb bones (humerus, ulna, and radius) in a Thai population. The sample consisted of 228 Thai skeletons (114 males and 114 females) from the Forensic Osteology Research Center, Chiang Mai University, Chiang Mai, Thailand. All skeletal length variables were measured according to the Standards for Data Collection from Human Skeletal Remains. The values of measurements were used to develop the sex estimation equations using univariate and multivariate discriminant function analyses. The results of sexual dimorphism showed that upper limb bone lengths of males were statistically significantly longer than those of females in all dimensions (P<0.05). For univariate discriminant function analysis, the results showed that the epicondylar breadth of the humerus, the physiological length of the ulna, and the antero-posterior diameter at the midshaft of the radius were the best indicators for sex estimation in the humerus, ulna, and radius, respectively. Moreover, the multivariate discriminant function equation using all variables of the ulna and radius was the best indicator for sex estimation. In conclusion, the discriminant function equations derived from upper limb bone measurements provided highly accurate sex estimation in Thai samples. Therefore, these equations using humerus, radius, and ulna measurements can be applied for sex estimation with good accuracy in Thais.
ABSTRACT
Nerve entrapment and blood circulation impairment associated with the medial side of the ankle are not uncommon. The purpose of this study was to describe the anatomical basis of neurovascular structures of the medial ankle which comprised the number, origin, branching pattern, and branching point. Forty feet of fresh cadavers were examined by using 2 reference lines: the malleolar-calcaneal (MC) and navicular-calcaneal (NC) axes. We recorded number, origin, length of the 2 axes, the locations and widths of neurovascular structures on MC and NC axes, the branching point of neurovascular structures, and the branching pattern of neurovascular structures was recorded and was separated into 5 types. The posterior tibial nerve (PTN) bifurcated to plantar and calcaneal nerves and branched proximally to the tarsal tunnel (TT). The posterior tibial artery bifurcated to plantar and calcaneal arteries and branched inferiorly to PTN and within the TT. The calcaneal nerves and arteries had more variation of number and origin. The most common branching point of calcaneal nerves and arteries is within the TT, except the medial calcaneal nerve. It branched proximally to the TT. The anatomical knowledge from this study is important for the diagnosis and treatment of clinicians.
ABSTRACT
Acromion is a major associated structure of subacromial impingement syndrome, one of the most common diagnoses in chronic shoulder pain world-wide. The aims of this study are to study morphometry of acromion and to find risk group using acromial morphometry. Total samples were 392 scapulae. The samples were of both sexes, ranging from the age of 31 to 90. Acromion type and osteophytes were observed. Acromial parameters were measured. The relationships were analyzed among acromion type, acromial osteophyte, acromial parameters, age group, sex and side. Curved acromion had the highest prevalence in all age groups. Hooked acromion had the second highest prevalence since the age of 41.Hooked acromion prevalence was higher in male than in female. The highest prevalence of acromial osteophytes was on anteroinferior surface in all age group. The prevalence of acromial osteophytes on anteroinferior surface and acromial facet increased with age. In addition, acromion type was associated with only osteophytes on anteroinferior surface of acromion.Anterior one-third acromial thickness in the age of 31 to 50 was different from those of 51 to 90. There are differences between all parameters and sexes, but not side. General population with age above 50 and concerned male group with age above 40 who have chronic shoulder pain should be investigated for subacromial impingement syndrome. Surgical treatment is recommended because hooked acromion and osteophytes are mostly the root of problem.